{"id":64,"date":"2019-06-15T20:37:42","date_gmt":"2019-06-15T11:37:42","guid":{"rendered":"http:\/\/www.fukui.bio.titech.ac.jp\/wordpress\/?page_id=64"},"modified":"2026-02-06T13:28:57","modified_gmt":"2026-02-06T04:28:57","slug":"publications","status":"publish","type":"page","link":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/publications\/","title":{"rendered":"Publications"},"content":{"rendered":"\n<h1 class=\"has-vivid-cyan-blue-color has-text-color\">\u7814\u7a76\u696d\u7e3e<\/h1>\n\n\n\n<hr class=\"wp-block-separator has-css-opacity is-style-wide\"\/>\n\n\n\n<h2>\u539f\u8457\u767a\u8868\u8ad6\u6587<\/h2>\n\n\n\n<ul>\n<li><a href=\"#kod\">\u8d85\u597d\u71b1\u30a2\u30fc\u30ad\u30a2\u306b\u95a2\u3059\u308b\u7814\u7a76<\/a><\/li>\n<\/ul>\n\n\n\n<ul>\n<li><a href=\"#pha\">\u751f\u5206\u89e3\u6027\u30dd\u30ea\u30de\u30fc\u306e\u5fae\u751f\u7269\u5408\u6210\u306b\u95a2\u3059\u308b\u7814\u7a76<\/a><\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-css-opacity is-style-wide\"\/>\n\n\n\n<h4 class=\"has-text-color\" id=\"kod\" style=\"color:#82878b\">\u8d85\u597d\u71b1\u30a2\u30fc\u30ad\u30a2\u306b\u95a2\u3059\u308b\u7814\u7a76<\/h4>\n\n\n\n<ul>\n<li>Pentose bisphosphate pathway can act in central metabolism for nucleoside-dependent growth of <em>Thermococcus kodakarensis<\/em> strains.<br><strong>Tetsu Nishida<\/strong>, <strong>Yangzi Chen<\/strong>, <strong>Takehiro Azuma<\/strong>, <strong>Izumi Orita<\/strong>, <strong>Toshiaki Fukui<\/strong><br><em>Appl. Environ. Microbiol.<\/em>, (2025)<br><a href=\"https:\/\/doi.org\/10.1128\/aem.01712-25\">https:\/\/doi.org\/10.1128\/aem.01712-25<\/a><\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Reversible RNA phosphorylation stabilizes tRNA for cellular thermotolerance.<br>Takayuki Ohira, Keiichi Minowa, Kei Sugiyama, Seisuke Yamashita, Yuriko Sakaguchi, Kenjyo Miyauchi, Ryo Noguchi, <strong>Akira Kaneko, Izumi Orita, Toshiaki Fukui,<\/strong> Kozo Tomita, Tsutomu Suzuki.<br><em>Nature.<\/em>, 605(7909), 372-379(2022)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Random mutagenesis of a hyperthermophilic archaeon identified tRNA modifications associated with cellular hyperthermotolerance.<br><strong>Izumi Orita<\/strong>, <strong>Ryohei Futatsuishi<\/strong>, <strong>Kyoko Adachi<\/strong>, Takayuki Ohira, <strong>Akira Kaneko<\/strong>, Keiichi Minowa, <strong>Miho Suzuki<\/strong>, <strong>Takeshi Tamura<\/strong>, Satoshi Nakamura, Tadayuki Imanaka, Tsutomu Suzuki, <strong>Toshiaki Fukui<\/strong>.<br><em>Nucleic Acids Res.<\/em>, 47, 1964-1976 (2019)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Genetic examination and mass balance analysis of pyruvate\/amino acid oxidation pathways in the hyperthermophilic archaeon <em>Thermococcus kodakarensis<\/em>.<br><strong>Kenta Nohara<\/strong>, <strong>Izumi Orita<\/strong>, Satoshi Nakamura, Tadayuki Imanaka, <strong>Toshiaki Fukui<\/strong>.<br><em>J. Bacteriol.<\/em>, 196, 3831-3839 (2014)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Characterization and gene deletion analysis of four homologues of group 3 pyridine nucleotide disulfide oxidoreductases from <em>Thermococcus kodakarensis<\/em>.<br><strong>Phurt Harnvoravongchai<\/strong>, <strong>Hiroki Kobori<\/strong>, <strong>Izumi Orita<\/strong>, Satoshi Nakamura, Tadayuki Imanaka, <strong>Toshiaki Fukui<\/strong>.<br><em>Extremophiles<\/em>, 18, 603-616 (2014)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Characterization of two members among the five ADP-forming acyl coenzyme A (acyl-CoA) synthetases reveals the presence of a  2-(imidazol-4-yl)acetyl-CoA synthetase in <em>Thermococcus kodakarensis<\/em>.<br>Tomotsugu Awano, Anja Wilming, Hiroya Tomita, Yuusuke Yokooji, <strong>Toshiaki Fukui<\/strong>, Tadayuki Imanaka, Haruyuki Atomi.<br><em>J. Bacteriol.<\/em>, 196, 140-147 (2014)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Thermostable alcohol dehydrogenase from <em>Thermococcus kodakarensis<\/em> KOD1 for enantioselective bioconversion of aromatic secondary alcohols.<br><strong>Xi Wu<\/strong>, Chong Zhang, <strong>Izumi Orita<\/strong>, Tadayuki Imanaka,  <strong>Toshiaki Fukui<\/strong>, Xin-Hui Xing.<br><em>Appl. Environ. Microbiol.<\/em>, 79, 2209-2217 (2013) <\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Overview of the genetic tools in the Archaea<br>Haruyuki Atomi, Tadayuki Imanaka, <strong>Toshiaki Fukui<\/strong>.<br><em>Front Microbiol.<\/em>, 3, 337 (2012)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Application of a novel thermostable NAD(P)H oxidase from hyperthermophilic archaeon for the regeneration of both NAD<sup>+<\/sup> and NADP<sup>+<\/sup>.<br><strong>Xi Wu<\/strong>, <strong>Hiroki Kobori<\/strong>, <strong>Izumi Orita<\/strong>, Chong Zhang, Tadayuki Imanaka, Xin-Hui Xing, <strong>Toshiaki Fukui<\/strong>.<br><em>Biotechnol. Bioeng.<\/em>, 109, 53-62 (2012) <\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Characterization of NADH Oxidase\/NADPH Polysulfide Oxidoreductase and  Its Unexpected Participation in Oxygen Sensitivity in an Anaerobic Hyperthermophilic Archaeon.<br><strong>Hiroki Kobori<\/strong>, <strong>Masayuki Ogino<\/strong>, <strong>Izumi Orita<\/strong>, Satoshi Nakamura, Tadayuki Imanaka, <strong>Toshiaki Fukui<\/strong>.<br><em>J. Bacteriol.<\/em>, 192, 5192-5202 (2010)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Methionine sulfoxide reductase from the hyperthermophilic archaeon <em>Thermococcus kodakaraensis<\/em>, an enzyme designed to function at sub-optimal growth temperatures.<br>Eiji Fukushima, Yasuhiro Shinka, <strong>Toshiaki Fukui<\/strong>, Haruyuki Atomi, Tadayuki Imanaka.<br><em>J. Bacteriol.<\/em>, 189, 7134-7144 (2007) <\/li>\n<\/ul>\n\n\n\n<ul>\n<li>A novel ADP-forming succinyl-CoA synthetase in <em>Thermococcus kodakaraensis<\/em> structurally related to the archaeal NDP-forming acetyl-CoA synthetases.<br>Kenichi Shikata, <strong>Toshiaki Fukui<\/strong>, Haruyuki Atomi, Tadayuki Imanaka.<br><em>J. Biol. Chem.<\/em>, 282, 26963-26970 (2007)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Disruption of a sugar transporter gene cluster in a hyperthermophilic  archaeon using a host\/marker system based on antibiotic resistance.<br>Rie Matsumi, Kenji Manabe, <strong>Toshiaki Fukui<\/strong>, Haruyuki Atomi, Tadayuki Imanaka.<br><em>J. Bacteriol.<\/em>, 189, 2683-2691 (2007)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Phosphoenolpyruvate synthase plays an essential role for glycolysis in the modified Embden-Meyerhof pathway in <em>Thermococcus kodakarensis<\/em>.<br>Hiroyuki Imanaka, Atsushi Yamatsu, <strong>Toshiaki Fukui<\/strong>, Haruyuki Atomi, Tadayuki Imanaka.<br><em>Mol. Microbiol.<\/em>, 61, 898-909 (2006)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li> Characterization of a novel glucosamine-6-phosphate deaminase from a hyperthermophilic archaeon.<br>Takeshi Tanaka, Fumikazu Takahashi,&nbsp;<strong>Toshiaki Fukui<\/strong>, Shinsuke Fujiwara, Haruyuki Atomi, Tadayuki Imanaka.<br><em>J. Bacteriol.<\/em>,&nbsp;187, 7038-7044 (2005) <\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-css-opacity is-style-wide\"\/>\n\n\n\n<h4 class=\"has-text-color\" id=\"pha\" style=\"color:#82878b\">\u751f\u5206\u89e3\u6027\u30dd\u30ea\u30de\u30fc\u306e\u5fae\u751f\u7269\u5408\u6210\u306b\u95a2\u3059\u308b\u7814\u7a76<\/h4>\n\n\n\n<ul>\n<li>Microaerobic insights into production of polyhydroxyalkanoates containing 3-hydroxyhexanoate via native reverse \u03b2-oxidation from glucose in <em>Ralstonia eutropha<\/em> H16.<br><strong>Kai-Hee Huong<\/strong>, <strong>Izumi Orita<\/strong>, <strong>Toshiaki Fukui<\/strong>.<br><em>Microbial Cell Factories<\/em>.,(2024)<br>https:\/\/doi.org\/10.1186\/s12934-024-02294-4<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Gas fermentation combined with water electrolysis for production of polyhydroxyalkanoate copolymer from carbon dioxide by engineered&nbsp;<em>Ralstonia eutropha<\/em>.<br><strong>Gabriele Di Stadio<\/strong>, <strong>Izumi Orita<\/strong>, Ryuhei Nakamura, <strong>Toshiaki Fukui<\/strong>.<br><em>Bioresource Technology<\/em>., (2024)<br>https:\/\/doi.org\/10.1016\/j.biortech.2023.130266<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>(<em>R<\/em>\/<em>S<\/em>)-lactate\/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by&nbsp;<em>Ralstonia eutropha<\/em>.<br><strong>Shizuru Ishihara<\/strong>,<strong>&nbsp;Izumi Orita<\/strong>, Ken\u2019ichiro Matsumoto,&nbsp;<strong>Toshiaki Fukui<\/strong>.<br><em>Appl. Microbiol. Biotechnol.<\/em>, (2023)<br>https:\/\/doi.org\/10.1007\/s00253-023-12797-6<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>C1\u5fae\u751f\u7269\u306e\u4ee3\u8b1d\u6539\u5909\u306b\u3088\u308b\u30e1\u30bf\u30ce\u30fc\u30eb\u3092\u539f\u6599\u3068\u3057\u305f\u751f\u5206\u89e3\u6027\u5171\u91cd\u5408\u30dd\u30ea\u30a8\u30b9\u30c6\u30eb\u306e\u751f\u5408\u6210<br><strong>Izumi Orita,<\/strong> <strong>Toshiaki Fukui.<\/strong><br><em>Journal of the Japan Petroleum Institute<\/em>., 65(6), 213-220(2022)<br>https:\/\/doi.org\/10.1627\/jpi.65.213<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Biosynthesis of Poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) From Glucose by <em>Escherichia coli<\/em> Through Butyryl-CoA Formation Driven by Ccr-Emd Combination.<br><strong>Shu Saito, Ryu Imai,<\/strong> Yuki Miyahara, <strong>Mari Nakagawa, Izumi Orita,<\/strong> Takeharu Tsuge, <strong>Toshiaki Fukui.<\/strong><br><em>Front Bioeng Biotechnol.<\/em>, 10:888973(2022)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Biosynthesis of Polyhydroxyalkanoate Terpolymer from Methanol via the Reverse \u03b2-Oxidation Pathway in the Presence of Lanthanide.<br><strong>Izumi Orita, Gento Unno, Risa Kato, Toshiaki Fukui.<\/strong><br><em>Microorganisms.<\/em>, 10(1), 184(2022)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Biosynthesis of Poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) from CO<sub>2<\/sub> by a Recombinant <em>Cupriavidusnecator<\/em>.<br>Kenji Tanaka, Kazumasa Yoshida, <strong>Izumi Orita, Toshiaki Fukui.<\/strong><br>Bioengineering (Basel)., 8(11), 179(2021)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis.<br>Ryoga Fujiwara, Rikako Sanuki, Hiroharu Ajiro, <strong>Toshiaki Fukui,<\/strong> Shosuke Yoshida.<br><em>Sci Rep.<\/em>, 11(1), 19991(2021)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Isopropanol production with reutilization of glucose-derived CO 2 by engineered <em>Ralstonia eutropha<\/em>.<br><strong>Dyah Candra Hapsari Subagyo, Rie Shimizu, Izumi Orita, Toshiaki Fukui.<\/strong><br><em>J Biosci Bioeng.,<\/em> 132(5), 479-486(2021)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Methylotrophic bacterium-based molecular sensor for the detection of low concentrations of methanol.<br><strong>Viviane Carnier Casaroli<\/strong>, <strong>Izumi Orita<\/strong>, Shiori Katayama, Hiroya Yurimoto, Yasuyoshi Sakai, <strong>Toshiaki Fukui<\/strong>.<br><em>J. Biosci Bioeng.<\/em>, 132(3), 247-252(2021)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>A study on the effects of increment and decrement repeated fed-batch feeding of glucose on the production of poly(3-hydroxybutyrate) [P(3HB)] by a newly engineered Cupriavidus necator NSDG-GG mutant in batch fill-and-draw fermentation.<br>Nazila Biglari, <strong>Izumi Orita<\/strong>, <strong>Toshiaki Fukui<\/strong>, Kumar Sudesh.<br><em>J. Biotechnol.<\/em>, 307, 77-86(2020)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds. <br><strong>Mengxiao Zhang<\/strong>, <strong>Shunsuke Kurita<\/strong>, <strong>Izumi Orita<\/strong>, Satoshi Nakamura, <strong>Toshiaki Fukui<\/strong>.<br> <em>Microb. Cell. Fact.<\/em>, 18, 147(2019) <\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Two NADH-dependent (<em>S<\/em>)-3-hydroxyacyl-CoA dehydrogenases from polyhydroxyalkanoate-producing <em>Ralstonia eutropha<\/em>.<br><strong>Mutsumi Segawa<\/strong>, <strong>Cheng Wen<\/strong>, <strong>Izumi Orita<\/strong>, Satoshi Nakamura, <strong>Toshiaki Fukui<\/strong>.<br><em>J. Biosci. Bioeng.<\/em>, 127, 294-300 (2019)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Enhancement of bioplastic polyhydroxybutyrate P(3HB) production from glucose by newly engineered strain <em>Cupriavidus necator<\/em> NSDG-GG using response surface methodology.<br>Nazila Biglari, Marjan Ganjali Dashti, Peyman Abdeshahian, <strong>Izumi Orita<\/strong>, <strong>Toshiaki Fukui<\/strong>, Kumar Sudesh.<br><em>3 Biotech<\/em>, 8, 330 (2018) <\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Compositional regulation of poly(3-hydroxybutyrate-<em>co<\/em>-3-hydroxyhexanoate) by replacement of granule-associated protein in <em>Ralstonia eutropha<\/em>.<br><strong>Yui Kawashima<\/strong>, <strong>Izumi Orita<\/strong>, Satoshi Nakamura, <strong>Toshiaki Fukui<\/strong>.<br><em>Microb. Cell. Fact.<\/em>, 14, 187 (2015)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>New Insight into the Role of the Calvin Cycle: Reutilization of CO<sub>2<\/sub> Emitted through Sugar Degradation.<br><strong>Rie Shimizu<\/strong>, Yudai Dempo, Yasumune Nakayama, Satoshi Nakamura, Takashi,bamba, Eiichiro Fukusaki, <strong>Toshiaki Fukui<\/strong>.<br><em>Sci. Rep.<\/em>, 10, 11617 (2015)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Improved artificial pathway for biosynthesis of poly(3-hydroxybutyrate-<em>co<\/em>-3-hydroxyhexanoate) with high C<sub>6<\/sub>-monomer composition from fructose in <em>Ralstonia eutropha<\/em>.<br><strong>Chayatip Insomphun<\/strong>, <strong>Huan Xie<\/strong>, <strong>Jun Mifune<\/strong>, <strong>Yui Kawashima<\/strong>, <strong>Izumi Orita<\/strong>, Satoshi Nakamura, <strong>Toshiaki Fukui<\/strong>.<br><em>Metab. Eng.<\/em>, 27, 38-45 (2015)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Enhancement of glycerol utilization ability of <em>Ralstonia eutropha<\/em> H16 for production of polyhydroxyalkanoates.<br><strong>Toshiaki Fukui<\/strong>, Masaharu Mukoyama, <strong>Izumi Orita<\/strong>, Satoshi Nakamura.<br><em>Appl. Microbiol. Biotechnol.<\/em>, 98,  7559-7568 (2014)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Biosynthesis of polyhydroxyalkanoate copolymers from methanol by <em>Methylobacterium extorquens<\/em> AM1 and the engineered strains under cobalt-deficient conditions.<br><strong>Izumi Orita<\/strong>,  <strong>Kouta Nishikawa<\/strong>, Satoshi Nakamura, <strong>Toshiaki Fukui<\/strong>.<br><em>Appl. Microbiol. Biotechnol.<\/em>, 98, 3715-3725 (2014)<\/li>\n<\/ul>\n\n\n\n<ul>\n<li>Modification of \u03b2-oxidation pathway in <em>Ralstonia eutropha<\/em> for production of poly(3-hydroxybutyrate-<em>co<\/em>-3-hydroxyhexanoate) from soybean oil.<br><strong>Chayatip Insomphun<\/strong>, <strong>Jun Mifune<\/strong>, <strong>Izumi Orita<\/strong>, Keiji Numata, Satoshi Nakamura, <strong>Toshiaki Fukui<\/strong>.<br><em>J. Biosci. Bioeng.<\/em>, 117, 184-190 (2014)<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u7814\u7a76\u696d\u7e3e \u539f\u8457\u767a\u8868\u8ad6\u6587 \u8d85\u597d\u71b1\u30a2\u30fc\u30ad\u30a2\u306b\u95a2\u3059\u308b\u7814\u7a76 \u751f\u5206\u89e3\u6027\u30dd\u30ea\u30de\u30fc\u306e\u5fae\u751f\u7269\u5408\u6210\u306b\u95a2\u3059\u308b\u7814\u7a76<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/pages\/64"}],"collection":[{"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/comments?post=64"}],"version-history":[{"count":54,"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/pages\/64\/revisions"}],"predecessor-version":[{"id":923,"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/pages\/64\/revisions\/923"}],"wp:attachment":[{"href":"http:\/\/www.fukui.life.isct.ac.jp\/index.php\/wp-json\/wp\/v2\/media?parent=64"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}